Revealing the diversity of algal monosaccharides: Fast carbohydrate fingerprinting of microalgae using crude biomass and showcasing sugar distribution in Chlorella vulgaris by biomass fractionation
作者： Jose G. Ortiz-Tena , Broder Rühmann , Doris Schieder , Volker Sieber
DOI: 10.1016/J.ALGAL.2016.05.008
关键词: Carbohydrate 、 Hydrolysis 、 Monosaccharide 、 Biomass 、 Chemistry 、 Sugar 、 Chlorella vulgaris 、 Chromatography 、 Biorefinery 、 Polysaccharide
摘要: The quantitative and qualitative analysis of carbohydrates from algae is essential for the optimal utilization algal biomass. A previously established high throughput method identification complex was applied to microalgae. It based on selective derivatization monosaccharides by 1-phenyl-3-methyl-5-pyrazolone (PMP), UHPLC separation MS derivatives. crude biomass six different representative microalgae analyzed monosaccharide composition after trifluoroacetic acid (TFA) hydrolysis reported here. In addition usually found neutral sugars, uronic acids, amino with this methylated phosphorylated or sulfated were also identified. PMP enabled a recovery in hydrolysates between 90% 105% five strains. Furthermore, fractionation process an overall 82% microalga Chlorella vulgaris investigate carbohydrate distribution cell. relative amounts starch, soluble associated glycoproteins glycolipids, as well structural polysaccharides determined. Due its advantages, can help strain screening designing proper strategies maximal development, formation rare sugars general emerging field biorefinery.
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