Separation and fractionation of exopolysaccharides from Porphyridium cruentum
作者： Anil Kumar Patel , Celine Laroche , Alain Marcati , Alina Violeta Ursu , Sébastien Jubeau
DOI: 10.1016/J.BIORTECH.2012.12.038
关键词:
摘要: In this work the extraction of EPSs from culture media Porphyridium cruentum, by dialysis, solvent-precipitation with 3 polar alcohols (methanol, ethanol and isopropanol) membrane separation techniques has been studied. Diafiltration (DF) using a 300 kDa molecular weight cut off was most efficient technique compared to solvent-extraction dialysis methods. After extraction, EPS fraction characterized in terms rheological properties biochemical content. The product exhibited shear thinning behavior critical overlap concentration equal 0.6 g/L. monosaccharide composition investigated after acidic hydrolysis. Xylose, galactose, glucose glucuronic acid were identified as main constitutive monomers.
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