Effective treatment of B16 melanoma by direct delivery of bleomycin using electrochemotherapy.

摘要: Electrochemotherapy (ECT), chemotherapy administered in combination with electric fields, has the potential to be an effective localized treatment for cutaneous malignancies. Bleomycin's cytotoxicity was enhanced by exposing tumour cells electrical fields following intravenous injection of chemotherapeutic agent. Two issues associated this procedure are existence a narrow but optimal time-window and fact that systemic drug dose is therapy. In order address these issues, study initiated examine effectiveness administering bleomycin intratumoural injection. A dose-response relationship determined. addition, minimal field strength necessary ECT established. Results indicated drastic reductions volume ECT-treated groups. groups showed increased survival over control The minimum intratumour group 0.025 units. found 1250 V/cm. results demonstrate pulses effective, information will used initiate clinical trials.