Protein Kinase C Is Required for Induction of 2′,5′-Oligoadenylate Synthetases☆

摘要: Abstract Induction of the p40/46 and p69/71 isoforms 2′,5′-oligoadenylate (2-5A) synthetase by interferon-α (IFN-α) is variable among six different Burkitt lymphoma cell lines with Ramos cells expressing highest levels these enzymes. Inhibitors protein kinase C (PKC) block induction mRNAs encoding both isoforms; however, isoform more sensitive to inhibitors. Downregulation PKC prolonged treatment 12-O-tetradecanoylphorbol-13-acetate (TPA) also blocks 2-5A decreases constitutive IFN-α-induced enzymatic activity. Cotreatment TPA IFN-α increases above that seen in treated alone. does not directly activate PKC-α or PKC-δ, two most abundant present cells, suggesting activation another signaling pathway necessary for maximal synthetases IFN-α.