Nucleotide sequence and analysis of the purA gene encoding adenylosuccinate synthetase of Escherichia coli K12.
作者： S A Wolfe , J M Smith
DOI: 10.1016/S0021-9258(18)37402-7
关键词: Adenylosuccinate 、 Primer extension 、 Gene 、 Dyad symmetry 、 Nucleic acid sequence 、 Start codon 、 Adenylosuccinate synthase 、 Molecular biology 、 Escherichia coli 、 Biology 、 Genetics
摘要: Abstract Adenylosuccinate synthetase (EC 6.3.4.4), encoded by the purA gene of Escherichia coli K12, catalyzes synthesis adenylosuccinate (SAMP) from IMP, first committed step in AMP biosynthesis. The E. K12 and flanking DNA was cloned miniMu-mediated transduction, nucleotide sequence determined. mature SAMP subunit, as deduced sequence, contains 427 amino acid residues has a calculated Mr 47,277. size mRNA determined Northern blotting to be approximately 1.5 kilobase pairs. 5'-end identified primer extension is located 23 nucleotides upstream ATG translational initiation codon. Comparison control region with guaBA revealed common dyad symmetry which may suggest mutual elements regulation. did not resemble regions other known pur loci.
sci-hub.st 参考文章(47)
MARK M. STAYTON, FREDERICK B. RUDOLPH, HERBERT J. FROMM, Regulation, genetics, and properties of adenylosuccinate synthetase: a review. Current Topics in Cellular Regulation. ,vol. 22, pp. 103- 141 ,(1983) , 10.1016/B978-0-12-152822-5.50008-7
G Millar, A Lewendon, M G Hunter, J R Coggins, The cloning and expression of the aroL gene from Escherichia coli K12. Purification and complete amino acid sequence of shikimate kinase II, the aroL-gene product. Biochemical Journal. ,vol. 237, pp. 427- 437 ,(1986) , 10.1042/BJ2370427
C A Makaroff, H Zalkin, Regulation of Escherichia coli purF. Analysis of the control region of a pur regulon gene. Journal of Biological Chemistry. ,vol. 260, pp. 10378- 10387 ,(1985) , 10.1016/S0021-9258(17)39258-X
Frederick Carl Neidhardt, John L Ingraham, K Brooks Low, Moselio Schaechter, Boris Magasanik, H Edwin Umbarger, Escherichia Coli and Salmonella: Typhimurium Cellular and Molecular Biology ,(1987)
J M Smith, H A Daum, Nucleotide sequence of the purM gene encoding 5'-phosphoribosyl-5-aminoimidazole synthetase of Escherichia coli K12. Journal of Biological Chemistry. ,vol. 261, pp. 10632- 10636 ,(1986) , 10.1016/S0021-9258(18)67432-0
P P Pang, A S Lundberg, G C Walker, Identification and characterization of the mutL and mutS gene products of Salmonella typhimurium LT2. Journal of Bacteriology. ,vol. 163, pp. 1007- 1015 ,(1985) , 10.1128/JB.163.3.1007-1015.1985
Jeffrey H Miller, Experiments in molecular genetics ,(1972)
A A Tiedeman, J M Smith, H Zalkin, Nucleotide sequence of the guaA gene encoding GMP synthetase of Escherichia coli K12. Journal of Biological Chemistry. ,vol. 260, pp. 8676- 8679 ,(1985) , 10.1016/S0021-9258(17)39400-0
R.K. Koduri, J.S. Gots, A DNA-binding protein with specificity for pur genes in Escherichia coli. Journal of Biological Chemistry. ,vol. 255, pp. 9594- 9598 ,(1980) , 10.1016/S0021-9258(18)43433-3
H. Aiba, S. Adhya, B. de Crombrugghe, Evidence for two functional gal promoters in intact Escherichia coli cells. Journal of Biological Chemistry. ,vol. 256, pp. 11905- 11910 ,(1981) , 10.1016/S0021-9258(19)68491-7