Nucleotide sequence of the guaA gene encoding GMP synthetase of Escherichia coli K12.

作者: A A Tiedeman , J M Smith , H Zalkin

DOI: 10.1016/S0021-9258(17)39400-0

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摘要: Abstract GMP synthetase (EC 6.3.4.1), a glutamine amido-transferase encoded by the guaA gene, catalyzes synthesis of from XMP. The gene was subcloned Clarke and Carbon (Clarke, L., Carbon, J. (1976) Cell 9, 91-99) plasmid pLC34-10, nucleotide sequence determined. structural encodes protein 525 amino acid residues having calculated Mr 58,604. NH2 terminus determined used to verify translation start site DNA sequence. A 68-base pair intercistronic region separates upstream guaB in polycistronic guaBA operon. 3' end mRNA S1 nuclease mapping. is 36-37 nucleotides downstream stop codon within dyad symmetry that resembles rho-independent transcription termination site.

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