High frequency of yeast transformation by plasmids carrying part of entire 2-μm yeast plasmid

作者: Claude Gerbaud , Philippe Fournier , Hughes Blanc , Michel Aigle , Henri Heslot

DOI: 10.1016/0378-1119(79)90080-5

关键词: Genomic libraryBiologyYeastPlasmid preparationTransformation efficiencyMolecular biologyPlasmidTransformation (genetics)T-DNA Binary systemURA3

摘要: … 180 mm X 18 mm test tubes andsubmersed in 3 mi of hybridization buffer containing 2 to 4 ~g … In conclusion, either hybridization or genetic studies did not give proof of integration of the …

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