Cloning and functional characterization of HKT1 and AKT1 genes of Fragaria spp.-Relationship to plant response to salt stress.

摘要: Commercial strawberry, Fragaria x ananassa Duch., is a species sensitive to salinity. Under saline conditions, Na(+) uptake by the plant increased, while K(+) significantly reduced. Maintaining an adequate K(+)/Na(+) cytosolic ratio determines ability of survive in environments. The goal present work was clone and functionally characterize genes AKT1 HKT1 involved transport strawberry determine relationship these with responses three spp. genotypes having different ecological adaptations salt stress. FaHKT1 FcHKT1 proteins from F. chiloensis have 98.1% identity, FaAKT1 FcAKT1 identity 99.7%. ananassa, were characterized Xenopus oocytes. FaHKT1, belongs group I HKT transporters selective for Na(+). Expression oocytes showed that protein typical inward-rectifying highly K(+)-selective channel. relative expression studied roots cv. Camarosa (accessions Bau Cucao) grown under transiently increased 'Camarosa', decreased 'Cucao' not affected 'Bau' upon other two genotypes. correlates higher tolerance salinity this genotype comparison 'Camarosa' 'Bau'.