The inward-rectifying K+ channel SsAKT1 is a candidate involved in K+ uptake in the halophyte Suaeda salsa under saline condition

摘要: The Shaker AKT1-like channels are considered to be involved in both high- and low-affinity K+ uptake correlated with salt tolerance glycophytes. Suaeda salsa (Suaeda maritima subsp. salsa), as a typical salt-accumulating halophyte, is able absorb efficiently while growing under saline conditions taking large amount of Na+, thus maintaining the concentration its cells. In this study, possible functions inward-rectifying channel SsAKT1 halophyte S. were investigated. from was isolated by RT-PCR characterized using yeast complementation; responses various KCl NaCl treatments investigated real-time quantitative PCR. consisted 879 amino acid residues shared high homology (60–67 %) identified AKT1 other plants. expression rescued K+-uptake-defective phenotype strain CY162, also suppressed salt-sensitive G19, suggesting functioned an channel. predominantly expressed roots, induced significantly starvation; transcript levels increased further on resupply (0.1–10 mM for 6 h) 62 % 0.1 mM K+ 144–174 % higher concentrations (1–10 mM). Interestingly, level roots short-term treatment (6 h) (25–250 mM). These results demonstrate that might mediate salsa, but play greater role system. Furthermore, participating maintenance nutrition salinity.