Recent advancements in structured-illumination microscopy toward live-cell imaging.

摘要: Fluorescence microscopy allows us to observe fluorescently labeled molecules in diverse biological processes and organelle structures within living cells. However, the diffraction limit restricts its spatial resolution about half of wavelength, limiting capability observation at molecular level. Structured-illumination (SIM), a type super-resolution microscopy, doubles all three dimensions by illuminating sample with patterned excitation light, followed computer reconstruction. SIM uses relatively low illumination power compared other methods is easily available for multicolor imaging. has great potential meeting requirements live-cell Recent developments types have achieved higher (∼50 nm lateral) temporal (∼100 Hz) resolutions. Here, we review recent advancements discuss application noninvasive