Changes in distribution, morphology, and tumor necrosis factor-alpha secretion of alveolar macrophage subpopulations during the development of bleomycin-induced pulmonary fibrosis.

摘要: Previous studies indicate that heterogeneous alveolar macrophages (AM) play a pivotal role in events associated with bleomycin-induced pulmonary fibrosis. A critical has been suggested for tumor necrosis factor-alpha (TNF-alpha), product of activated macrophages, this fibrotic process. The present study examined whether the characteristics and function (TNF-alpha secretion) rat AM subpopulations were altered during development After intratracheal bleomycin treatment, separated into 18 density-defined subpopulations. Bleomycin treatment distribution morphology densities 1.017 to 1.061 g/ml at all time points studied (4, 14, 28 days). Subpopulations 1.090 1.141 affected only 4 days after treatment. Tumor secretion increased 14- 28-day samples bleomycin-treated rats, particularly 1.075 1.097 g/ml. These data alterations distribution, morphology, accompany When coupled previous suggesting TNF-alpha plays process disease model, these are responsible production