Phosphoprotein Keratin 23 accumulates in MSS but not MSI colon cancers in vivo and impacts viability and proliferation in vitro
作者： Karin Birkenkamp-Demtroder , Francisco Mansilla , Flemming Brandt Sørensen , Mogens Kruhøffer , Teresa Cabezón
DOI: 10.1016/J.MOLONC.2007.05.005
关键词: Phosphoprotein 、 Keratin 、 Molecular biology 、 Colorectal cancer 、 Cell growth 、 Biology 、 Adenocarcinoma 、 Programmed cell death 、 Immunohistochemistry 、 Cancer research 、 CEBPB
摘要: Transcript profiling of 27 normal colon mucosas and 258 adenocarcinomas showed Keratin23 to be increased in 78% microsatellite-stable tumors, while microsatellite-instable tumors low transcript levels, comparable mucosas. Immunohistochemical analyses demonstrated that 88% were negative for protein, 70% MSS metastases derived from MSS-tumors high levels. Immunofluorescence analysis localized the Golgi-apparatus. Golgi accumulation was unique gastrointestinal adenocarcinomas. Immunoprecipitation 2D-blot revealed a 46.8 kDa phosphoprotein. impaired proliferation human cancer cells significantly, leading cell death but not lines, COS7 experienced multiple nuclei apoptosis. expression correlated significantly with transcription factor CEBPB. In conclusion, is novel important difference between cancers.
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