Repression of KIAA1199 attenuates Wnt-signalling and decreases the proliferation of colon cancer cells.
作者： K Birkenkamp-Demtroder , A Maghnouj , F Mansilla , K Thorsen , C L Andersen
DOI: 10.1038/BJC.2011.268
关键词:
摘要: The KIAA1199 transcript is upregulated in colon adenomas and downregulated upon β-catenin knockdown. Transcript profiling was performed on >500 biopsies, methylation data were compared with data. Immunohistochemistry assessed protein expression 270 stage II/III tumours (>3 years follow-up). effects of stable knockdown SW480 cells (three different constructs) studied using transcriptional profiling, proliferation analysis. strongly 95% adenocarcinomas. Absent normal mucosa correlated promotor methylation. Nuclear cytoplasmic identified adenocarcinomas other types cancers. A subpopulation patients expressing the nucleus showed a better outcome regard to recurrence as lung or liver metastases. affected cell cycle Wnt-signalling pathway. Reduced cellular decreased KI67, phosphorylated retinoblastoma, ASCL2 supported these findings. Eighteen genes differentially expressed profile clinical specimens. attenuates Wnt/β-catenin signalling it may thus be regarded regulatory part this
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