The Role of miRNAs in Cisplatin-Resistant HeLa Cells

摘要: Chemotherapy is the main strategy in treatment of cancer, however, development drug-resistance obstacle long-term cervical cancer. Cisplatin one most common drugs used cancer therapy. Recently, accumulating evidence suggests that miRNAs are involved various bioactivities oncogenesis. It not unexpected play a key role acquiring progression tumor. In this study, we induced and maintained four levels cisplatin-resistant HeLa cell lines (HeLa/CR1, HeLa/CR2, HeLa/CR3 HeLa/CR4). According to previous studies exiting evidence, selected five (miR-183, miR-182, miR-30a, miR-15b miR-16) their potential target mRNAs as our research targets. The real-time RT-PCR was detect relative expression mRNAs. results show miR-182 were up-regulated resistant while miR-30a significantly down-regulated. At same time, targets they regulated related drug-resistance. alteration compared parent line drug resistance associated with distinct miRNAs, which indicates may be therapy by sensitizing chemotherapy.