KIAA1199 as a potential diagnostic biomarker of rheumatoid arthritis related to angiogenesis.
作者： Xinyu Yang , Pengcheng Qiu , Bingbing Chen , Yaoyao Lin , Zhonghao Zhou
DOI: 10.1186/S13075-015-0637-Y
关键词:
摘要: Our previous proteomic study on fibroblast-like synoviocytes (FLSs) derived from the synovial tissues found that expression of KIAA1199 was higher in rheumatoid arthritis (RA) patients than healthy controls. The aim this to examine biological function and evaluate its clinical diagnosis value RA. over-expression verified by quantitative real-time polymerase chain reaction (qPCR), Immunohistochemistry, Immunofluorescence enzyme linked immunosorbent assay (ELISA) inactive active RA effect FLSs proliferation, angiogenesis related pathway were analyzed MTT, cell migration, tube formation, chorioallantoic membrane (CAM) assay, qPCR western-blotting after knockdown over-expression. verification results show up-regulation at mRNA protein level as compared ELISA receiver operator characteristic (ROC) analysis shows concentration serum, fluid could be used dependable biomarkers for RA, provided an area under roc curve (AUC) 0.83, 0.92 0.92. Sensitivity specificity, which determined cut-off points, reached 72% 84% 80% sensitivity 80%, 93.3%, 93.3% respectively. Moreover, also enhance proliferation membrane, KIAA1199/ PLXNB3/ SEMA5A/CTGF axis may a newly enhancing angiogenesis. potential diagnostic biomarker
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