Detection of Wolbachia Infections in Natural and Laboratory Populations of the Moroccan Hessian Fly, Mayetiola destructor (Say).

作者: Naima Bel Mokhtar , Amal Maurady , Mohammed Reda Britel , Mustapha El Bouhssini , Costas Batargias

DOI: 10.3390/INSECTS11060340

关键词: BiologyBiological pest controlStrain (biology)PopulationZoologyDNA sequencingPEST analysisMayetiola destructorPhylogenetic treeWolbachia

摘要: Mayetiola destructor (Hessian fly) is a destructive pest of wheat in several parts the world. Here, we investigated presence reproductive symbionts and effect geographical location on bacterial community associated to adult Hessian flies derived from four major producing areas Morocco. Using specific 16S rDNA PCR assay, Wolbachia infection was observed 3% natural populations 10% laboratory population. High throughput sequencing V3-V4 region rRNA gene revealed that microbiota significantly influenced by their native regions. A total 6 phyla, 10 classes 79 genera were obtained all samples. Confirming screening results, identified as well flies. Phylogenetic analysis using sequences this study indicated there one strain belonging supergroup A. To our knowledge, first report fly populations. The low abundance most likely does not indicate induction incompatibility. Yet, may give new insight into use for fight against

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