Distinct microbial populations exist in the mucosa-associated microbiota of sub-groups of irritable bowel syndrome.

摘要: Background  There is increasing evidence to support a role for the gastrointestinal microbiota in etiology of irritable bowel syndrome (IBS). Given an inflammatory component IBS, mucosa-associated potentially play key its pathogenesis. The objectives were compare between patients with diarrhea predominant IBS (IBS-D), constipation (IBS-C) and controls using fluorescent situ hybridization correlate specific bacteria groups individual symptoms. Methods  Forty-seven (27 IBS-D 20 IBS-C) 26 healthy recruited study. Snap-frozen rectal biopsies taken at colonoscopy bacterial quantification performed by hybridizing frozen sections bacterial-group oligonucleotide probes. Key Results  Patients had significantly greater numbers total per mm epithelium than [median 218 (IQR – 209) vs 128 (121) P = 0.007], this was chiefly comprised bacteroides [69 (67) 14 (41) P = 0.001] Eubacterium rectale–Clostridium coccoides [52 (58) 25 (35) P = 0.03]. Analysis sub-groups demonstrated that bifidobacteria lower group IBS-C [24 (32) 54 (88) 32 P = 0.011]. Finally, amongst maximum number stools day negatively correlated (P < 0.001) lactobacilli (P = 0.002). Conclusions & Inferences  different from increases clostridia reduction IBS-D.