Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations.

摘要: Fluorescent oligonucleotide hybridization probes were used to label bacterial cells for analysis by flow cytometry. The probes, complementary short sequence elements within the 16S rRNA common phylogenetically coherent assemblages of microorganisms, labeled with tetramethylrhodamine and hybridized suspensions fixed cells. Flow cytometry was resolve individual target nontarget bacteria (1 5 microns) via probe-conferred fluorescence. Target quantified in an excess intensity fluorescence increased additively combined use two or three fluorescent different regions same rRNA. Images