Purified DNAs are transcribed after microinjection into Xenopus oocytes.

摘要: The possibility of using DNA-injected Xenopus laevis oocytes and eggs for studying the control transcription in eukaryotes has been investigated. When purified DNA simian virus 40 (SV40) is injected into oocytes, tritiated RNA precursors are incorporated DNase-I-resistant, RNase-A- alkali-sensitive material that hybridizes specifically to SV40 DNA. This viral continues at least 5 days occurs only when directed nucleus oocyte. quantity SV40-specific produced roughly proportional amount injected; above 1 ng per oocyte, most nonribosomal made successfully virus-specific. Transcription also occurs, although a lower efficiency, after injection unfertilized eggs. DNAs adenovirus 5, cloned Drosophila melanogaster histone genes, even bacteriophage phiX174 replicative form, phi80plac, ColE1 plasmid transcribed or