Rapid, modified oxidase test for oxidase-variable bacterial isolates.
作者： J J Tarrand , D H Gröschel
DOI: 10.1128/JCM.16.4.772-774.1982
关键词: Reagent 、 Microbiology 、 Dimethyl sulfoxide 、 Bacteria 、 Agar 、 Aqueous solution 、 Oxidase test 、 Biology
摘要: A modified oxidase reagent, 1% tetramethyl-p-phenylenediamine in dimethyl sulfoxide, proved superior to the routinely used aqueous dihydrochloride detecting weakly oxidase-positive gram-negative bacteria after 24 h of growth on agar media (40 40 positive versus 22 positive). The bacterial inoculum was obtained with a cotton-tipped swab instead loop or wooden applicator, and reaction required less than 15 s.
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