Modified oxidase and benzidine tests for separation of staphylococci from micrococci.

摘要: Two simple and rapid methods for the separation of staphylococci from micrococci are described. They based on modified oxidase benzidine tests. Micrococci Staphylococcus sciuri yield a blue color with 6% solution tetramethylphenylenediamine in dimethyl sulfoxide, whereas all other exhibit no coloration. Best's results were obtained overnight cultures blood agar. The presence c-type cytochromes i S. could be detected benzidine; noncovalently linked heme groups removed before addition reagent. test is simplest most method (except sciuri) micrococci, if nutritional requirements time incubation strictly followed. This especially recommended examination clinical material which usually not found.