Fast Induction of High-Affinity HCO3− Transport in Cyanobacteria
作者： Dieter Sültemeyer , Barbara Klughammer , Murray R Badger , G Dean Price , None
DOI: 10.1104/PP.116.1.183
关键词: Biochemistry 、 De novo synthesis 、 Staurosporine 、 Enzyme 、 Synechococcus 、 Ion transporter 、 Okadaic acid 、 Cyanobacteria 、 Carbonic anhydrase 、 Chemistry
摘要: The induction of a high-affinity state the CO 2 -concentration mechanism was investigated in two cyanobacterial species, Synechococcus sp. strain PCC7002 and PCC7942. Cells grown at high concentrations were resuspended low-CO buffer illuminated presence carbonic anhydrase for 4 to 10 min until inorganic C compensation point reached. Thereafter, more than 95% induced both species. Mass-spectrometric analysis HCO 3 − fluxes indicated that only affinity transport increased during fast-induction period, whereas maximum activities not affected. kinetic characteristics uptake remained unchanged. Fast inhibited by chloramphenicol, cantharidin, or okadaic acid. In contrast, fast did occur K252a, staurosporine, genistein, which are known inhibitors protein kinases. These results show can within minutes exposure low-inorganic-C conditions may involve posttranslational phosphorylation existing proteins rather de novo synthesis new components.
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oup.com参考文章(39)
H. P. Fock, D. F. Sültemeyer, O2 Evolution and Uptake Measurements in Plant Cells by Mass Spectrometry Modern Methods of Plant Analysis. pp. 3- 18 ,(1989) , 10.1007/978-3-642-83346-5_1
Flavio Meggio, Arianna Donella Deana, Maria Ruzzene, Anna M. Brunati, Luca Cesaro, Barbara Guerra, Thomas Meyer, Helmut Mett, Doriano Fabbro, Pascal Furet, Grazyna Dobrowolska, Lorenzo A. Pinna, Different susceptibility of protein kinases to staurosporine inhibition Kinetic studies and molecular bases for the resistance of protein kinase CK2 FEBS Journal. ,vol. 234, pp. 317- 322 ,(1995) , 10.1111/J.1432-1033.1995.317_C.X
Aaron Kaplan, Rakefet Schwarz, Judy Lieman-Hurwitz, Michal Ronen-Tarazi, Leonora Reinhold, Physiological and Molecular Studies on the Response of Cyanobacteria to Changes in the Ambient Inorganic Carbon Concentration Springer, Dordrecht. pp. 469- 485 ,(1994) , 10.1007/978-94-011-0227-8_15
Y. Matsuda, B. Colman, Induction of CO2 and Bicarbonate Transport in the Green Alga Chlorella ellipsoidea (I. Time Course of Induction of the Two Systems) Plant Physiology. ,vol. 108, pp. 247- 252 ,(1995) , 10.1104/PP.108.1.247
P. N. Tyrrell, R. A. Kandasamy, C. M. Crotty, G. S. Espie, Ethoxyzolamide Differentially Inhibits CO2 Uptake and Na+-Independent and Na+-Dependent HCO3- Uptake in the Cyanobacterium Synechococcus sp. UTEX 625. Plant Physiology. ,vol. 112, pp. 79- 88 ,(1996) , 10.1104/PP.112.1.79
A.G. Miller, D.T. Canvin, Distinction between HCO3−- and CO2-dependent photosynthesis in the cyanobacteriumSynechococcus leopoliensisbased on the selective response of HCO3−transport to Na+ FEBS Letters. ,vol. 187, pp. 29- 32 ,(1985) , 10.1016/0014-5793(85)81207-2
Walter Maier, Karl Hammer, Ulrike Dammann, Barbara Schulz, Dieter Strack, Accumulation of sesquiterpenoid cyclohexenone derivatives induced by an arbuscular mycorrhizal fungus in members of the Poaceae Planta. ,vol. 202, pp. 36- 42 ,(1997) , 10.1007/S004250050100
Stephen A. Bloye, Nigel J. Silman, Nicholas H. Mann, Noel G. Carr, Bicarbonate concentration by Synechocystis PCC6803 : modulation of protein phosphorylation and inorganic carbon transport by glucose Plant Physiology. ,vol. 99, pp. 601- 606 ,(1992) , 10.1104/PP.99.2.601
Murray R. Badger, G. Dean Price, The CO2 concentrating mechanism in cyanobacteria and microalgae Physiologia Plantarum. ,vol. 84, pp. 606- 615 ,(1992) , 10.1034/J.1399-3054.1992.840416.X
Anthony G. Miller, George S. Espie, David T. Canvin, Active Transport of CO2 by the Cyanobacterium Synechococcus UTEX 625 : Measurement by Mass Spectrometry Plant Physiology. ,vol. 86, pp. 677- 683 ,(1988) , 10.1104/PP.86.3.677