Influence of para-substituents on the oxidative metabolism of o-nitrophenols by Pseudomonas putida B2.

摘要: Pseudomonas putida B2 is able to grow on o-nitrophenol (ONP) as the sole source of carbon and nitrogen. ONP was converted by a nitrophenol oxygenase nitrite catechol. Catechol then attacked catechol 1,2-dioxygenase further degraded through an ortho-cleavage pathway. derivatives which were para-substituted with methyl-, chloro-, carboxy-, formyl- or nitro-group failed support growth strain B2. Relevant catabolic enzymes characterized analyze why these not mineralized. Nitrophenol soluble, NADPH-dependent enzyme that stimulated magnesium, manganese, calcium ions. It active toward ONP, 4-methyl-, 4-chloro-, lesser extent, 4-formyl-ONP but 4-carboxy- 4-nitro-ONP. In addition, 4-formyl-, 4-carboxy-, 4-nitro-ONP induce formation oxygenase. 4-methyl-catechol only poorly chlorinated catechols. 4-Methyl-catechol likely be methyl-lactones, are often dead-end metabolites in bacteria. Thus, compounds tested, unsubstituted acts inducer substrate for all productive