A Changeable Lab-on-a-Chip Detector for Marine Nonindigenous Microorganisms in Ship’s Ballast Water

作者: Myint Maw , Xinxiang Pan , Zhen Peng , Yanjuan Wang , Long Zhao

DOI: 10.3390/MI9010020

关键词: Water qualityBiological systemLab-on-a-chipDetectorMicrochannelMicrofluidic chipEnvironmental scienceElectrical impedanceMicroorganismBallast

摘要: The spread and invasion of many nonindigenous species in the ship’s ballast water around world has been a hazard threat to ecology, economy, human health. rapid accurate detection marine invasive is essential. This article aimed at analysing quality by means changeable microfluidic chip detector thus comply with D-2 standard management sediment convention. system was designed through integration technology, impedance pulse sensing LED light induced chlorophyll fluorescence (LED-LICF) detection. can measure number, size, shape, volume targeted microorganisms, it also determine intensity, which an important factor activity phytoplankton. samples were Chlorella volutis, Dunaliella salina, Platymonas subcordiformis, Chrysophytes, Escherichia coli, Enterococci. whole or operation be accomplished online few minutes using micron sample solution. valid data outputs are simultaneously displayed terms both amplitudes fluorescent intensity signals. for multi-sizes real time changing microchannel sizes on chip. Because successfully detect label-free applicable in-situ detections some modifications system.

参考文章(33)
Will Anderson, Rebecca Lane, Darren Korbie, Matt Trau, Observations of Tunable Resistive Pulse Sensing for Exosome Analysis: Improving System Sensitivity and Stability. Langmuir. ,vol. 31, pp. 6577- 6587 ,(2015) , 10.1021/ACS.LANGMUIR.5B01402
Junsheng Wang, Younan Song, Myint Myint Maw, Yongxin Song, Xinxiang Pan, Yeqing Sun, Dongqing Li, Detection of size spectrum of microalgae cells in an integrated underwater microfluidic device Journal of Experimental Marine Biology and Ecology. ,vol. 473, pp. 129- 137 ,(2015) , 10.1016/J.JEMBE.2015.08.016
Jun Zhang, Sheng Yan, Dan Yuan, Gursel Alici, Nam-Trung Nguyen, Majid Ebrahimi Warkiani, Weihua Li, Fundamentals and applications of inertial microfluidics: a review Lab on a Chip. ,vol. 16, pp. 10- 34 ,(2016) , 10.1039/C5LC01159K
Darby Kozak, Will Anderson, Robert Vogel, Matt Trau, Advances in resistive pulse sensors: Devices bridging the void between molecular and microscopic detection Nano Today. ,vol. 6, pp. 531- 545 ,(2011) , 10.1016/J.NANTOD.2011.08.012
David Alsteens, Heykel Trabelsi, Patrice Soumillion, Yves F. Dufrêne, Multiparametric atomic force microscopy imaging of single bacteriophages extruding from living bacteria Nature Communications. ,vol. 4, pp. 2926- ,(2013) , 10.1038/NCOMMS3926
Allen C. S. Yu, Jacky F. C. Loo, Samuel Yu, S. K. Kong, Ting-Fung Chan, Monitoring bacterial growth using tunable resistive pulse sensing with a pore-based technique Applied Microbiology and Biotechnology. ,vol. 98, pp. 855- 862 ,(2014) , 10.1007/S00253-013-5377-9
Li Du, Jiang Zhe, Joan Carletta, Robert Veillette, Fred Choy, Real-time monitoring of wear debris in lubrication oil using a microfluidic inductive Coulter counting device Microfluidics and Nanofluidics. ,vol. 9, pp. 1241- 1245 ,(2010) , 10.1007/S10404-010-0627-Y
Vendula Pospichalova, Jan Svoboda, Zankruti Dave, Anna Kotrbova, Karol Kaiser, Dobromila Klemova, Ladislav Ilkovics, Ales Hampl, Igor Crha, Eva Jandakova, Lubos Minar, Vit Weinberger, Vitezslav Bryja, Simplified protocol for flow cytometry analysis of fluorescently labeled exosomes and microvesicles using dedicated flow cytometer. Journal of extracellular vesicles. ,vol. 4, pp. 25530- 25530 ,(2015) , 10.3402/JEV.V4.25530
C. Constantinescu, Trends and challenges in VLSI circuit reliability IEEE Micro. ,vol. 23, pp. 14- 19 ,(2003) , 10.1109/MM.2003.1225959
Dan-Qun HUO, Zhen LIU, Chang-Jun Hou, Jun YANG, Xiao-Gang LUO, Huan-Bao FA, Jia-Le DONG, Yu-Chan ZHANG, Guo-Ping ZHANG, Jun-Jie LI, Recent Advances on Optical Detection Methods and Techniques for Cell-based Microfluidic Systems Chinese Journal of Analytical Chemistry. ,vol. 38, pp. 1357- 1365 ,(2010) , 10.1016/S1872-2040(09)60067-0