Contractile and Structural Reactions to Impediments of Ca2+-Homeostasis in Physarum polycephalum
作者： Wohlfarth Bottermann , Z. Shraideh , Z. Baranowski
DOI: 10.1247/CSF.8.255
关键词: Physarum polycephalum 、 Ultrastructure 、 Biophysics 、 Anatomy 、 Contraction (grammar) 、 Isometric exercise 、 Biology 、 Calcium 、 Cytoplasm 、 Actin 、 Fibril
摘要: A combined application of 5 mM KCN and 19 microM Ca++-ionophore A-23187 leads to pronounced contractures plasmodial strands Physarum polycephalum. The appearance the is independent amount Ca++ in external medium. Tensiometric registrations longitudinal contraction activity (isometric regime) reveal an average tension increase 50 mp compared with preceding level before addition ionophore. This high force output during contracture coincides a number cytoplasmic actomyosin fibrils. Their ultrastructure seen as lateral density strictly parallel arranged F-actin filaments; state this isometric corresponds isometrically contracted fibrils normal contraction-relaxation cycle organism. simultaneous impediment respiration homeostasis strongly favours shift actin equilibrium polymeric side form may thus be used preparatory step improving specimens context other investigations, e.g., for immunocytochemical investigations or preparation cell-free models reactivated after extraction procedures.
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