Identification of apoptosis and macrophage migration events in paraquat-induced oxidative stress using a zebrafish model

摘要: Abstract Aims Paraquat (PQ) is a pesticide highly toxic to human beings, and well-known trigger of oxidative stress. Although several animal models PQ poisoning have been developed, some disadvantages limit their application in vivo. A zebrafish model was used the present study better define mechanisms stress injury induced by PQ. Main methods The toxicity evaluated AB strain zebrafish, apoptosis assessed acridine orange staining. Macrophage migration identified using TG ( zlyz : EGFP ) transgenic strain, angiogenesis observed fli1a-EGFP casper strain. Following validation gene changes zebrafish-based vivo quantitative real-time PCR, network analysis performed Ingenuity Pathway Analysis software. Key findings We first established LC 50 model, then found that robust antioxidant genes were activated after exposure. Moreover, distinct macrophage activation for time PQ-exposed zebrafish. Utilizing this both extrinsic intrinsic pathways involved PQ-induced elucidated. further demonstrated specifically PQ, Rho family members JNK-MMP13 signaling participated process. Significance Zebrafish promising tool investigating screening effective anti-oxidant drugs.