Determinants and consequences of different levels of CagA phosphorylation for clinical isolates of Helicobacter pylori

摘要: Abstract Background & Aims: The Helicobacter pylori cag pathogenicity island encodes a secretory system that translocates CagA into epithelial cells, where it becomes tyrosine phosphorylated and induces cytoskeletal rearrangements. Strains with more phosphorylation motifs are most closely associated gastric cancer. Here we assess whether clinical strains can deliver CagA, different numbers of have to degrees, this amounts change. Methods: Forty-four H. from South African patients, all cagA gene positive, were cocultured the adenocarcinoma cell line AGS. expression determined by Western blot interleukin-8 secretion enzyme-linked immunosorbent assay. 3′ variable regions 22 sequenced shown possess 3–6 motifs. These used quantify Results: genotype typing genes poorly predictive phenotype. Thirty-four 44 expressed protein could be delivered within AGS cells. Only these 34 induced Among those strains, number degree level biologic activity in terms extent elongation. Conclusions: induce higher levels changes, likely