Cadherin switching in ovarian cancer progression

摘要: The roles of the cadherins in progression ovarian cancer to late stages disease state when malignant cells have disseminated within peritoneal cavity remain poorly understood. In view these observations, we undertaken a comprehensive survey cadherin subtypes present normal surface epithelium and peritoneum tumors effusions women diagnosed with Stage I or II primary using degenerate cloning strategy for sequences highly conserved among this family cell adhesion molecules. On basis nucleotide resultant PCR products, multiple (E-, N-, P-cadherin, cadherin-4, -6, -11) were found be tissues cells. P-cadherin was determined predominant subtype peritoneum, tumor masses. An increase mRNA protein expression levels masses later confirmed by Northern Western blot analysis, respectively. addition, that cadherin-associated protein, known as beta-catenin, is expressed obtained from cancer. Immunoprecipitation studies demonstrated capable interacting beta-catenin Collectively, findings suggest regulated P-cadherin/beta-catenin complexes may represent key step progression.