TRPC3 properties of a native constitutively active Ca2+‐permeable cation channel in rabbit ear artery myocytes

摘要: Previously we have described a constitutively active, Ca2+-permeable, non-selective cation channel in freshly dispersed rabbit ear artery myocytes which has similar properties to some of the canonical transient receptor potential (TRPC) proteins. In present work compared constitutive activity with known TRPC proteins by investigating effect selective anti-TRPC antibodies and pharmacological agents on whole-cell single activity. Bath application anti-TRPC3 markedly reduced inside-out patches also produced pronounced reduction both current amplitude variance active currents whereas anti-TRPC1/4/5/6/7 had no presence antigenic peptide, or flufenamic acid, Gd3+, La3+ Ca2+ inhibited spontaneous outside-out IC50 values 6.8 μm, 25 nm, 1.5 μm 0.124 mm, respectively, are those against TRPC3 Immunocytochemical studies combined confocal microscopy showed expression myocytes, these were predominately distributed at, close to, plasma membrane. These data provide strong evidence that native channels indicate may an important role mediating this conductance.