Human fatty acid synthase mRNA: tissue distribution, genetic mapping, and kinetics of decay after glucose deprivation.

摘要: To better understand the accelerated decay of fatty acid synthase (FAS) message that occurs after glucose deprivation (J. Biol. Chem. 1993. 268: 6961-6970), we characterized 3' terminus human and kinetics FAS mRNA in HepG2 cells. The gene was localized to chromosome 17q24-25 syntenic distal mouse 11. Expression tissues ubiquitous with high levels liver, lung, intra-abdominal adipose tissue. 806 nucleotide untranslated region contained two regions instability pentamer AUUUA. Unlike short-lived messages containing AUUUA motifs, not first order, there were no detectable changes poly(A) tail. Glucose transiently caused sediment more rapidly than control density gradients. In vivo treatment different translational inhibitors showed translation per se necessary for decay; association polysomes protected it from decay. cell-free experiments, rapid using components glucose-deprived glucose-treated These data suggest regulates cytoplasmic stability by partitioning between a translated pool subject degradation compartment, features reminiscent regulated other diet-responsive messages.