RNA polymerase backtracking drives the accumulation of fission yeast condensin at active genes
作者： Julieta Rivosecchi , Laetitia Vachez , François Gautier , Pascal Bernard , Vincent Vanoosthuyse
DOI: 10.1101/2020.11.13.381434
关键词: Biology 、 Cell biology 、 Condensin 、 Polymerase 、 RNA polymerase 、 Mitosis 、 Transcription (biology) 、 RNA 、 DNA 、 Gene
摘要: The mechanisms leading to the accumulation of SMC complex condensin around specific transcription units in mitosis remain unclear. Observations made bacteria suggested that RNA polymerases (RNAP) constitute an obstacle translocation, particularly when RNAP and travel opposite directions. Whether this also applies eukaryotic remains Here we show fission yeast focused at 39 end RNAP2-transcribed gene after flipping its orientation, suggesting termini harbor intrinsic condensin-positioning features whatever orientation transcription. Consistent with this, provide evidence termination position involved. Moreover, stabilize backtracked RNAP2 within bodies was sufficient cancel redistribute it evenly units. Altogether, our results suggest backtracking, which is frequent termini, plays a key role positioning strengthen idea dense arrays proteins tightly-bound DNA alter distribution on mitotic chromosomes.
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