Effect of epithelial debridement on glycosaminoglycan synthesis by human corneal explants
作者： Eduardo S Soriano , Mauro SQ Campos , Yara M Michelacci , None
DOI: 10.1016/S0009-8981(00)00188-1
关键词: Biochemistry 、 Chondroitin sulfate B 、 Sulfate 、 Dermatan sulfate 、 Heparan sulfate 、 Glycosaminoglycan 、 Chemistry 、 Keratan sulfate 、 Cell culture 、 Tissue culture
摘要: Abstract The purpose of the present work was to investigate effects mechanical epithelial debridement upon glycosaminoglycan synthesis by human corneal explants. Corneal explants were maintained under tissue culture conditions for 2–72 days and glycosaminoglycans synthesized in 24 h metabolically labeled addition 35 S-sulfate medium. These compounds isolated from analyzed a combination agarose gel electrophoresis enzymatic degradation with specific mucopolysaccharidases. cells corneas previously submitted cell compared controls. Keratan sulfate (26 kDa) dermatan (43 main glycosaminoglycans, each one corresponding about 50% total. Nevertheless, S-labeled S-dermatan (73%), smaller amounts S-keratan (15%) S-heparan (12%), suggesting lower rate keratan sulfate. heparan removal layer caused decrease labeling induced stromal cells. This increased suggests relationship between epithelium stroma could be related opacity that may appear after debridement.
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