Polyethylene glycol improves phenol removal by immobilized turnip peroxidase
作者： F. Quintanilla-Guerrero , M.A. Duarte-Vázquez , B.E. García-Almendarez , R. Tinoco , R. Vazquez-Duhalt
DOI: 10.1016/J.BIORTECH.2008.04.031
关键词: Chromatography 、 Substrate (chemistry) 、 Peroxidase 、 Effluent 、 Calcium alginate 、 Phenols 、 PEG ratio 、 Polyethylene glycol 、 Phenol 、 Chemistry
摘要: Purified peroxidase from turnip (Brassica napus L. var. esculenta D.C.) was immobilized by entrapment in spheres of calcium alginate and covalent binding to Affi-Gel 10. Both Turnip (TP) preparations were assayed for the detoxification a synthetic phenolic solution real wastewater effluent local paints factory. The effectiveness compounds (PC's) removal oxidative polymerization evaluated using batch recycling processes, presence absence polyethylene glycol (PEG). PEG enhances operative TP stability. In addition, reaction times reduced 3h 10 min, more effective phenol removals achieved when added. able perform 15 cycles with industrial showing PC's >90% during first cycles. High efficiencies (>95%) obtained both at concentrations <1.2mM. Higher decreased efficiency 90% after cycle, probably due substrate inhibition. On other hand, showed increased thermal stability compared free TP. A large-scale enzymatic process treatment is expected be developed that could stable enough make economically feasible.
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