Impacts of fluorescent superparamagnetic iron oxide (SPIO)-labeled materials on biological characteristics and osteogenesis of bone marrow mesenchymal stem cells (BMSCs).
作者： Bin Ren , Zhenwen Na , Xin Zhao , Weixian Liu , Guangping Zhang
DOI:
关键词: Pathology 、 Cell 、 Biophysics 、 Staining 、 Alkaline phosphatase 、 Mesenchymal stem cell 、 Cytoplasm 、 Medicine 、 Osteocalcin 、 Bone sialoprotein 、 RUNX2
摘要: The aim of this study was to investigate the impacts fluorescent superparamagnetic iron oxide particles (Molday ION Rhodamine B, MIRB) on bioactivities and osteogenetic differentiation rat bone marrow mesenchymal stem cells (BMSCs). Cell Counting Kit-8 (CCK-8) method used detect proliferation (SPIO)-labeled BMSCs observed distribution MIRB in cells; real time -polymerase chain reaction (RT-PCR) analyze expressions such osteogenesis-related genes as sialoprotein, alkaline phosphatase (ALP), RUNX2, bonemorphogeneticprotein-2 (BMP-2), type 1 collagen (COL-1) 3 (COL-3); ALP-Alizarin red staining poly-biochemical analyzer were qualitatively quantitatively metabolites. labeled distributed cytoplasm BMSCs, diameter larger could be up several hundred nanometers, concentrated around nuclei, far away from nuclei smaller, but labeled-cells' skeletons adherent morphology did not change significantly; under concentration 25 μg Fe/mL of, affect cellular viabilities gene ALP, RUNX2 BMP-2 decreased, secretion amount ALP osteocalcin also declined. would cell structures SPIO aggregated formed granules which might osteogenesis BMSCs.
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