Single-labeled oligonucleotide probes for homogeneous nucleic acid sequence analysis
作者： Carl T. Wittwer , Noriko Kusukawa , Wade Stevenson , Andrew O. Crockett , Brian E. Caplin
DOI:
关键词: Nucleic acid sequence 、 Molecular beacon 、 Chemistry 、 Oligonucleotide 、 Fluorescence 、 Genotyping 、 Molecular biology 、 Biochemistry 、 Melting curve analysis 、 Nucleic acid 、 Homogeneous
摘要: Probes and methods are provided for detection analysis of nucleic acid sequences. The probes single-labeled oligonucleotide whose fluorescence emission changes in response to probe-target hybridization dissociation. analyzing one or multiple loci using the probes. This invention further relates use melting curve analysis, genotyping, pathogen detection, quantification specific sequences real-time monitoring amplification.
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