Detection of PCR products using self-probing amplicons and fluorescence.

摘要: Molecular diagnostics is progressing from low-throughput, heterogeneous, mostly manual technologies to higher throughput, closed-tube, and automated methods. Fluorescence the favored signaling technology for such assays, a number of techniques rely on energy transfer between fluorophore proximal quencher molecule. In these methods, dual-labeled probes hybridize an amplicon changes in quenching are detected. We describe new that simple use, gives highly specific information, avoids major difficulties alternative It uses primer with integral tail used probe extension product primer. The probing target sequence thereby converted into unimolecular event, which has substantial benefits terms kinetics, thermodynamics, assay design, reliability.