New Technologies for Sensitive and Specific Routine Detection of Plant Pathogenic Bacteria

摘要: In the last decade, nucleic acid sequence based amplification methods have been developed for many important plant pathogenic bacteria. Although they possess excellent properties with respect to sensitivity and specificity, introduction of these techniques routine detection has hampered by in particular lack robustness. different ring tests, both human pathogens, failures PCR diagnose correctly infected non-infected sample material demonstrated times. Carry-over contamination amplicons recognized as main source false-positive results, whereas presence inhibiting components extracts is known reason false-negative results. this paper new technologies approaches are described improve quality DNA RNA detection.