Seed Transcriptome Annotation Reveals Enhanced Expression of Genes Related to ROS Homeostasis and Ethylene Metabolism at Alternating Temperatures in Wild Cardoon

摘要: The association among environmental cues, ethylene response, ABA signaling, and reactive oxygen species (ROS) homeostasis in the process of seed dormancy release is nowadays well-established many species. Alternating temperatures are recognized as one main signals determining release, but their underlying mechanisms scarcely known. Dry after-ripened wild cardoon achenes germinated poorly at a constant temperature 20, 15, or 10 °C, whereas germination was stimulated by 80% alternating 20/10 °C. Using an RNA-Seq approach, we identified 23,640 annotated 14,078 gene transcripts expressed dry exposed to temperatures. Transcriptional patterns condition included reserve response dehydration stress genes (i.e., HSPs, peroxidases, LEAs). At temperature, observed upregulation biosynthesis NCED9), ABA-responsive ABI5 TAP), well other previously related physiological inhibition germination. However, were associated with metabolism ACO1, 4, ACS10) signaling EXPs) ROS regulators RBOH CAT). Accordingly, production twice high than presence medium synthesis inhibitors reduced significantly, not completely, Conversely, methyl viologen salicylhydroxamic acid (SHAM), peroxidase inhibitor, partially increased temperature. Taken together, present study provides first insights into expression (Cynara cardunculus var. sylvestris).