Circ_0002711 knockdown suppresses cell growth and aerobic glycolysis by modulating miR-1244/ROCK1 axis in ovarian cancer.

摘要: It has been well investigated that circular RNAs (circRNAs) play important roles in various cancers. The function of circ_0002711 and its underlying mechanisms ovarian cancer (OC) remain unknown. qRT-PCR western blot were performed to detect the expressions circ_0002711, microRNA-1244 (miR-1244), Rho kinase 1 (ROCK1) OC tissues cells. MTT assay colony formation employed evaluate cell proliferation. Detection lactate production, glucose uptake, ATP level oxygen consumption used determine Warburg effect. Western was examine glycolysis or proliferationrelated genes. Dual-luciferase reporter RIP pull down address relationship among miR-1244, ROCK1. In vivo tumor growth evaluated nude mice. Circ_0002711 upregulated lines. downregulation inhibited viability, ability aerobic glycolysis. contained binding sites with miR1244. Moreover, loss miR-1244 undermined downregulation-mediated function. ROCK1 miR-1244. MiR-1244 upregulation suppressed proliferation glycolysis, which rescued by enhanced expression knockdown hampered upregulating expression. Finally, decreased vivo. Circ_0002711/miR-1244/ROCK1 axis regulated effect