Pro-apoptotic effect of Persea americana var. Hass (avocado) on Jurkat lymphoblastic leukemia cells.
作者： Angelica R. Bonilla-Porras , Andrea Salazar-Ospina , Marlene Jimenez-Del-Rio , Andres Pereañez-Jimenez , Carlos Velez-Pardo
DOI: 10.3109/13880209.2013.842599
关键词: Biology 、 Jurkat cells 、 Apoptosis 、 Ethidium bromide 、 Hass avocado 、 Biochemistry 、 Leukemia 、 Persea 、 Acridine orange 、 Staining
摘要: AbstractContext: Therapy for leukemia has a limited efficacy. There is need to search alternative anti-leukemia therapies. Persea americana Mill var. Hass (Lauraceae) tropical fruit (avocado) that might be used against cancer.Objective: To investigate whether P. induces death in Jurkat lymphoblastic cells.Materials and methods: Four ethanol extracts (0.1, 0.5, 1, 2 5 mg/mL) from avocado (endocarp, whole seed, seed leaves) were analyzed cells. Hydrogen peroxide generation by oxidation of 2′,7′-dichlorodihydrofluorescein diacetate the fluorescent compound 2′,7′-dichlorfluorescein assay, acridine orange/ethidium bromide staining, flow cytometry analysis annexin-V/7-amino-actinomycin, mitochondrial membrane potential immunocytochemistry detection transcription factor p53, caspase-3 apoptosis-inducing (AIF) evaluated.Results: Endocarp, leaf (0.1 mg/mL) induced significant apoptosis c...
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