Direct electrochemistry and electrocatalysis of hemoglobin protein entrapped in graphene and chitosan composite film.

作者: Huifeng Xu , Hong Dai , Guonan Chen

DOI: 10.1016/J.TALANTA.2009.12.006

关键词: ChitosanBiomoleculeChemistryElectrochemistryNanotechnologyElectrocatalystGrapheneBiosensorElectron transferComposite number

摘要: Abstract A novel, biocompatible sensing strategy based on graphene and chitosan composite film for immobilizing the hemoglobin protein was firstly adopted. The direct electron transfer bioelectrocatalytic activity of after incorporation into were investigated. pair reversible redox waves appeared, could exhibit its toward H2O2 in a long term. Such results indicated that be friendly interface biomolecules keeping their native structure. Furthermore, appearance facilitate between matrix electroactive center hemoglobin. Hence, this protocol would promising platform immobilization biosensor preparation.

参考文章(22)
J.E.T. 22. Karlberg, B., Grasserbauer, M. and Andersen, European Analytical Column : Analytical chemistry faces challenges Trends in Analytical Chemistry. ,vol. 28, pp. 515- 518 ,(2009) , 10.1016/J.TRAC.2009.04.002
Ralph A Kamin, George S Wilson, None, Rotating ring-disk enzyme electrode for biocatalysis kinetic studies and characterization of the immobilized enzyme layer Analytical Chemistry. ,vol. 52, pp. 1198- 1205 ,(1980) , 10.1021/AC50058A010
Victor Glezer, Ovadia Lev, Sol-gel vanadium pentaoxide glucose biosensor Journal of the American Chemical Society. ,vol. 115, pp. 2533- 2534 ,(1993) , 10.1021/JA00059A072
Jue Lu, Lawrence T. Drzal, Robert M. Worden, Ilsoon Lee, Simple Fabrication of a Highly Sensitive Glucose Biosensor Using Enzymes Immobilized in Exfoliated Graphite Nanoplatelets Nafion Membrane Chemistry of Materials. ,vol. 19, pp. 6240- 6246 ,(2007) , 10.1021/CM702133U
Steffen Kröning, Frieder W. Scheller, Ulla Wollenberger, Fred Lisdat, Myoglobin-Clay Electrode for Nitric Oxide (NO) Detection in Solution Electroanalysis. ,vol. 16, pp. 253- 259 ,(2004) , 10.1002/ELAN.200402780
Ying Liu, Xiaohu Qu, Hongwei Guo, Hongjun Chen, Baifeng Liu, Shaojun Dong, Facile preparation of amperometric laccase biosensor with multifunction based on the matrix of carbon nanotubes-chitosan composite. Biosensors and Bioelectronics. ,vol. 21, pp. 2195- 2201 ,(2006) , 10.1016/J.BIOS.2005.11.014
Jenny S. Long, Debbie S. Silvester, Gregory G. Wildgoose, Annette-E. Surkus, Gerd-Uwe Flechsig, Richard G. Compton, Direct electrochemistry of horseradish peroxidase immobilized in a chitosan–[C4mim][BF4] film: Determination of electrode kinetic parameters Bioelectrochemistry. ,vol. 74, pp. 183- 187 ,(2008) , 10.1016/J.BIOELECHEM.2008.07.008
Jing Li, Shaojum Guo, Yueming Zhai, Erkang Wang, Nafion–graphene nanocomposite film as enhanced sensing platform for ultrasensitive determination of cadmium Electrochemistry Communications. ,vol. 11, pp. 1085- 1088 ,(2009) , 10.1016/J.ELECOM.2009.03.025
Maogen Zhang, Audrey Smith, Waldemar Gorski, Carbon Nanotube−Chitosan System for Electrochemical Sensing Based on Dehydrogenase Enzymes Analytical Chemistry. ,vol. 76, pp. 5045- 5050 ,(2004) , 10.1021/AC049519U