Hydrogen Peroxide Sensor Based on Coimmobilized Methylene Green and Horseradish Peroxidase in the Same Montmorillonite-Modified Bovine Serum Albumin−Glutaraldehyde Matrix on a Glassy Carbon Electrode Surface

摘要: A new approach to construct a second-generation amperometric biosensor is described. The classical dye methylene green as probing-needle mediator and horseradish peroxidase base enzyme were coimmobilized in the same montmorillonite-modified bovine serum albumin (BSA)−glutaraldehyde matrix H2O2 sensor. immobilization was formed from pretreated sodium montmorillonite colloid which cross-linker dissolved. Immobilization of mother solution attributed adsorption function montmorillonite, whereas cross-linking BSA−glutaraldehyde usual. Cyclic voltammetry potentiostatic measurements indicated that efficiently mediated electrons electrode matrix. sensor responded rapidly low concentration achieved 95% steady-state current less than 20 s, with a...