Optimized design of a nanostructured SPCE-based multipurpose biosensing platform formed by ferrocene-tethered electrochemically-deposited cauliflower-shaped gold nanoparticles.
作者： Wicem Argoubi , Maroua Saadaoui , Sami Ben Aoun , Noureddine Raouafi
DOI: 10.3762/BJNANO.6.187
关键词: Cyclic voltammetry 、 Nanotechnology 、 Selectivity 、 Bioconjugation 、 Colloidal gold 、 Dielectric spectroscopy 、 Ferrocene 、 Combinatorial chemistry 、 Electrochemistry 、 Biosensor 、 Materials science
摘要: The demand for on-site nanodevices is constantly increasing. technology development the design of such devices highly regarded. In this work, we report a disposable platform that structured with cauliflower-shaped gold nanoparticles (cfAuNPs) and show its applications in immunosensing enzyme-based detection. electrochemical reduction Au(III) allows electrodeposition dispersed on surface screen-printed carbon electrodes (SPCEs). nanostructures were functionalized using ferrocenylmethyl lipoic acid ester which allowed tethering ferrocene group to gold, serves as an transducer/mediator. bioconjugation anti-human IgG antibody (α-hIgG) or horseradish peroxidase (HRP) enzyme yields biosensors, have been applied selective detection human (hIgG) H2O2 model analytes, respectively. Parameters number sweeps, amount charge generated from oxidation electrodeposited time incubation concentration derivatives studied cyclic voltammetry (CV), impedance spectroscopy (EIS) scanning electron microscopy (SEM). Selectivity specificity tests also performed presence potentially interfering substances either hIgG H2O2. Results showed devised immunosensor endowed good selectivity several folds competitive analytes. catalytic activity towards predestined it potential pertaining enzymatic kinetics studies. levels serum honey successfully determined served assessment tools applicability platforms real samples analysis.
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