Enhanced cartilage tissue engineering by sequential exposure of chondrocytes to FGF-2 during 2D expansion and BMP-2 during 3D cultivation.
作者： Ivan Martin , R. Suetterlin , W. Baschong , M. Heberer , G. Vunjak-Novakovic
DOI: 10.1002/JCB.1203
关键词: Transforming growth factor beta 、 Fibroblast 、 Bone morphogenetic protein 2 、 Scaffold 、 Chemistry 、 Fibroblast growth factor 、 Anatomy 、 Glycosaminoglycan 、 Chondrocyte 、 Immunostaining 、 Cell biology
摘要: Bovine calf articular chondrocytes, either primary or expanded in monolayers (2D) with without 5 ng/ml fibroblast growth factor-2 (FGF-2), were cultured on three-dimensional (3D) biodegradable polyglycolic acid (PGA) scaffolds 10 bone morphogenetic protein-2 (BMP-2). Chondrocytes FGF-2 exhibited high intensity immunostaining for smooth muscle alpha-actin (SMA) and collagen type I induced shrinkage of the PGA scaffold, thus resembling contractile fibroblasts. presence 6 weeks yielded engineered cartilage 3.7-fold higher cell number, 4.2-fold wet weight, 2.8-fold weight glycosaminoglycan (GAG) fraction than chondrocytes FGF-2. BMP-2 similar cellularity size, 1.5-fold GAG fraction, more homogenous distribution corresponding BMP-2. The during 3D culture had no apparent effect those In summary, 2D expansion reduced chondrocyte expression fibroblastic molecules responsiveness to cultivation scaffolds.
sci-hub.se 参考文章(35)
G Gabbiani, T Seemayer, O Skalli, R Lagacé, W Schürch, B Pittet, D Montandon, Myofibroblasts from diverse pathologic settings are heterogeneous in their content of actin isoforms and intermediate filament proteins. Laboratory Investigation. ,vol. 60, pp. 275- 285 ,(1989)
MARION AHRENS, THOMAS ANKENBAUER, DIETMAR SCHRÖDER, ANGELA HOLLNAGEL, HUBERT MAYER, GERHARD GROSS, Expression of Human Bone Morphogenetic Proteins-2 or -4 in Murine Mesenchymal Progenitor C3H10T½ Cells Induces Differentiation into Distinct Mesenchymal Cell Lineages DNA and Cell Biology. ,vol. 12, pp. 871- 880 ,(1993) , 10.1089/DNA.1993.12.871
G Gabbiani, I Darby, O Skalli, Alpha-smooth muscle actin is transiently expressed by myofibroblasts during experimental wound healing Laboratory Investigation. ,vol. 63, pp. 21- 29 ,(1990)
KLAUS VON DER MARK, VERENA GAUSS, HELGA VON DER MARK, PETER MÜLLER, Relationship between cell shape and type of collagen synthesised as chondrocytes lose their cartilage phenotype in culture Nature. ,vol. 267, pp. 531- 532 ,(1977) , 10.1038/267531A0
G. Gabbiani, G. B. Ryan, G. Majno, Presence of modified fibroblasts in granulation tissue and their possible role in wound contraction. Cellular and Molecular Life Sciences. ,vol. 27, pp. 549- 550 ,(1971) , 10.1007/BF02147594
I. Martin, G. Vunjak-Novakovic, J. Yang, R. Langer, L.E. Freed, Mammalian chondrocytes expanded in the presence of fibroblast growth factor 2 maintain the ability to differentiate and regenerate three-dimensional cartilaginous tissue. Experimental Cell Research. ,vol. 253, pp. 681- 688 ,(1999) , 10.1006/EXCR.1999.4708
A. J. Celeste, J. A. Iannazzi, R. C. Taylor, R. M. Hewick, V. Rosen, E. A. Wang, J. M. Wozney, Identification of transforming growth factor beta family members present in bone-inductive protein purified from bovine bone Proceedings of the National Academy of Sciences of the United States of America. ,vol. 87, pp. 9843- 9847 ,(1990) , 10.1073/PNAS.87.24.9843
G. Vunjak-Novakovic, I. Martin, B. Obradovic, S. Treppo, A. J. Grodzinsky, R. Langer, L. E. Freed, Bioreactor cultivation conditions modulate the composition and mechanical properties of tissue-engineered cartilage. Journal of Orthopaedic Research. ,vol. 17, pp. 130- 138 ,(1999) , 10.1002/JOR.1100170119
Stefan Nehrer, Howard A. Breinan, Arun Ramappa, Sonya Shortkroff, Gretchen Young, Tom Minas, Clement B. Sledge, Ioannis V. Yannas, Myron Spector, Canine chondrocytes seeded in type I and type II collagen implants investigated in vitro. Journal of Biomedical Materials Research. ,vol. 38, pp. 95- 104 ,(1997) , 10.1002/(SICI)1097-4636(199722)38:2<95::AID-JBM3>3.0.CO;2-B
A P Hollander, T F Heathfield, C Webber, Y Iwata, R Bourne, C Rorabeck, A R Poole, Increased damage to type II collagen in osteoarthritic articular cartilage detected by a new immunoassay. Journal of Clinical Investigation. ,vol. 93, pp. 1722- 1732 ,(1994) , 10.1172/JCI117156