Mammalian chondrocytes expanded in the presence of fibroblast growth factor 2 maintain the ability to differentiate and regenerate three-dimensional cartilaginous tissue.

摘要: The differentiated phenotype of chondrocytes from hyaline cartilage is gradually lost during expansion in monolayers. Chondrocytes can reexpress their by transfer into an environment that prevents cell flattening, but serially passaged cells never completely recover chondrogenic potential. We report expanded (up to 2000-fold) the presence fibroblast growth factor 2 (FGF-2) dedifferentiated, fully maintained potential for redifferentiation response environmental changes. After seeding onto three-dimensional polymer scaffolds, FGF-2 formed cartilaginous tissue was histologically and biochemically comparable obtained using primary chondrocytes, contrast same degree absence FGF-2. inhibited formation thick F-actin structures, which otherwise monolayer expansion, were cultivation, associated with reduced ability phenotype. This study provides evidence maintains chondrocyte monolayers, possibly due changes architecture elements allows more efficient utilization harvested engineering.