Separation and quantification of cysteine, glutathione and phytochelatins in rice (Oryza sativa L.) upon cadmium exposure using reverse phase ultra performance liquid chromatography (RP-UPLC) with fluorescence detection†

摘要: Thiol compounds in plants, such as cysteine (Cys), glutathione (GSH) and phytochelatins (PCs), may be induced upon exposure to excessive potentially toxic metals metalloids. In this paper, Cys, GSH, PC2, PC3 PC4 rice leaf, stem root samples were derived with monobromobimane (mBBr), separated by reverse phase-ultra performance liquid chromatography (RP-UPLC) detected fluorescence. The results showed that, after reduction tris(β-chloroethyl)phosphate (TCEP), the above thiols rapidly binary gradient elution 15 min. calibration curve was linear a range of 1–20 μg mL−1. limit detection (LOD) for 0.14 pmol 1 μL injection (0.10 g−1 F.W), recovery 91.55% on average. Compared previously reported methods, technique developed work demonstrated higher speed sensitivity simultaneous measurements GSH PCs rice. Upon μM Cd 7 days, PC2–4 remarkably roots, whereas amounts Cys dominant thiol leaves stems, reduced.