Robust method for the analysis of phytochelatins in rice by high-performance liquid chromatography coupled with electrospray tandem mass spectrometry based on polymeric column materials

摘要: A sensitive and robust high-performance liquid chromatography coupled with electrospray tandem mass spectrometry method for the identification quantification of glutathione phytochelatins from rice was developed. Homogenized samples were extracted water containing 100 mM dithiothreitol, solid-phase extraction using polymer anion exchange resin employed sample purification. Chromatography performed on a polymeric column acetonitrile 0.1% formic acid as mobile phase at flow rate 300 μL/min. The limit quantitation 6-100 nM. This assay showed excellent linearity both over physiological normal ranges, correlation coefficients (r) > 0.9976. Recoveries four biothiols within range 76-118%, relative standard deviations less than 15%. intraday precision (n = 7) 2.1-13.3%, interday 15 days 4.3-15.2%. optimized applied to analyze tissue grown nutrient solutions three different cadmium concentrations (0, 50, μM). With increasing concentrations, content phytochelatin 2 3 in roots increased, contrast most phytochelatins, stems decreased significantly.