Modulation of arachidonic acid distribution by conjugated linoleic acid isomers and linoleic acid in MCF-7 and SW480 cancer cells.

摘要: The relationship between growth and alterations in arachidonic acid (AA) metabolism human breast (MCF-7) colon (SW480) cancer cells was studied. Four different fatty preparations were evaluated: a mixture of conjugated linoleic (CLA) isomers (c9,t11, t10,c12,c11,t13, minor amounts other isomers), the pure c9,t11-CLA isomer, t10,c12-CLA (LA) (all at lipid concentration 16 μg/mL). 14C-AA uptake into monoglyceride fraction MCF-7 significantly increased following 24 h incubation with CLA (P<0.05) (P<0.02). In contrast to cells, triglyceride SW480 while phospholipids reduced treatment (P<0.02) (P<0.05). Distribution among phospholipid classes altered by treatments both cell lines. isomer decreased phosphatidylcholine increasing phosphatidylethanolamine Both (P<0.01) phosphatidylserine but had no effect on this cells. Release derivatives not LA line. inhibited conversion 14C-prostaglandin E2 (PGE2) 20–30% 14C-PGF2α 17–44% relative controls 14C-PGD2 13–19% lines 14C-PGE2 20% line only. 5-hydroperoxyeicosatetraenoate 27% Lipid peroxidation, as determined levels 8-epi-prostaglandin F2α (8-epi-PGF2α), observed only These data indicate that growth-promoting effects may be associated enhanced AA PGE2 growth-suppressing due changes distribution cellular lipids an prostaglandin profile.