Effects of matrix proteins on rabbit corneal epithelial cell adhesion and migration.

作者: J. D. Cameron , S. T. Hagen , R. R. Waterfield , L. T. Furcht

DOI: 10.3109/02713688809047035

关键词: Cell biologyIntegrinBiochemistryType IV collagenCell adhesion moleculeBiologyBasement membraneCell adhesionLamininFibronectinExtracellular matrix

摘要: Extracellular matrix and basement membrane proteins are important in promoting the adhesion migration of various cell types. Laminin type IV collagen found membranes throughout body while fibronectin is associated with some basal lamina, loose connective tissue, all fluids. In current study fibronectin, laminin, were assessed to determine their effect on dissociated rabbit epithelial cells. a kinetic analysis was be most effective three adhesion. Cell promoted by could modulated an arg-gly-asp-ser (RGDS) peptide, sequence which within molecule. dose-response experiments effectively at concentrations as low 4×10–16 moles per well 96 tissue culture plates. Type protein tested chemotactic and...

参考文章(20)
A H Neufeld, M M Jumblatt, Beta-adrenergic and serotonergic responsiveness of rabbit corneal epithelial cells in culture. Investigative Ophthalmology & Visual Science. ,vol. 24, pp. 1139- 1143 ,(1983)
ELLIOTT SCHIFFMANN, JOHN I. GALLIN, Biochemistry of phagocyte chemotaxis. Current Topics in Cellular Regulation. ,vol. 15, pp. 203- 261 ,(1979) , 10.1016/B978-0-12-152815-7.50010-7
D E Smith, D F Mosher, R B Johnson, L T Furcht, Immunological identification of two sulfhydryl-containing fragments of human plasma fibronectin. Journal of Biological Chemistry. ,vol. 257, pp. 5831- 5838 ,(1982) , 10.1016/S0021-9258(19)83854-1
D E Smith, L T Furcht, Localization of two unique heparin binding domains of human plasma fibronectin with monoclonal antibodies. Journal of Biological Chemistry. ,vol. 257, pp. 6518- 6523 ,(1982) , 10.1016/S0021-9258(20)65172-9
SL Rogers, JB McCarthy, SL Palm, LT Furcht, PC Letourneau, Neuron-specific interactions with two neurite-promoting fragments of fibronectin The Journal of Neuroscience. ,vol. 5, pp. 369- 378 ,(1985) , 10.1523/JNEUROSCI.05-02-00369.1985
K Watanabe, S Nakagawa, T Nishida, Stimulatory Effects of Fibronectin and EGF on Migration of Corneal Epithelial Cells Investigative Ophthalmology & Visual Science. ,vol. 28, pp. 205- 211 ,(1987)
R. Timpl, H. Rohde, P.G. Robey, S.I. Rennard, J.M. Foidart, G.R. Martin, Laminin--a glycoprotein from basement membranes. Journal of Biological Chemistry. ,vol. 254, pp. 9933- 9937 ,(1979) , 10.1016/S0021-9258(19)83607-4
Hynda K. Kleinman, Mary L. McGarvey, Lance A. Liotta, Pamela Gehron Robey, Karl Tryggvason, George R. Martin, Isolation and characterization of type IV procollagen, laminin, and heparan sulfate proteoglycan from the EHS sarcoma. Biochemistry. ,vol. 21, pp. 6188- 6193 ,(1982) , 10.1021/BI00267A025
Teruo Nishida, Fibronectin. A new therapy for corneal trophic ulcer Archives of Ophthalmology. ,vol. 101, pp. 1046- 1048 ,(1983) , 10.1001/ARCHOPHT.1983.01040020048007
L S Fujikawa, C S Foster, I K Gipson, R B Colvin, Basement membrane components in healing rabbit corneal epithelial wounds: immunofluorescence and ultrastructural studies. Journal of Cell Biology. ,vol. 98, pp. 128- 138 ,(1984) , 10.1083/JCB.98.1.128