High-throughput isolation of immunoglobulin genes from single human B cells and expression as monoclonal antibodies.
作者： Hua-Xin Liao , Marc C. Levesque , Ashleigh Nagel , Ashlyn Dixon , Ruijun Zhang
DOI: 10.1016/J.JVIROMET.2009.02.014
关键词: B cell 、 Immunoglobulin gene 、 Gene expression 、 Virology 、 Cell culture 、 Molecular biology 、 Monoclonal antibody 、 Gene 、 Biology 、 Cloning 、 Antibody
摘要: Defining human B cell repertoires to viral pathogens is critical for design of vaccines that induce broadly protective antibodies to infections such as HIV-1 and influenza. Single B cell …
cabdirect.org
elsevier.com
sci-hub.se 参考文章(37)
R. Küppers, M. Zhao, M.L. Hansmann, K. Rajewsky, Tracing B cell development in human germinal centres by molecular analysis of single cells picked from histological sections. The EMBO Journal. ,vol. 12, pp. 4955- 4967 ,(1993) , 10.1002/J.1460-2075.1993.TB06189.X
David C. Montefiori, Evaluating Neutralizing Antibodies Against HIV, SIV, and SHIV in Luciferase Reporter Gene Assays Current protocols in immunology. ,vol. 64, ,(2004) , 10.1002/0471142735.IM1211S64
R I Brezinschek, H P Brezinschek, P E Lipsky, Analysis of the heavy chain repertoire of human peripheral B cells using single-cell polymerase chain reaction. Journal of Immunology. ,vol. 155, pp. 190- 202 ,(1995)
Susumu Tonegawa, Somatic generation of antibody diversity Nature. ,vol. 302, pp. 575- 581 ,(1983) , 10.1038/302575A0
Xiaowei Wang, B.David Stollar, Human immunoglobulin variable region gene analysis by single cell RT-PCR Journal of Immunological Methods. ,vol. 244, pp. 217- 225 ,(2000) , 10.1016/S0022-1759(00)00260-X
Hua-Xin Liao, Laura L. Sutherland, Shi-Mao Xia, Mary E. Brock, Richard M. Scearce, Stacie Vanleeuwen, S. Munir Alam, Mildred McAdams, Eric A. Weaver, Zenaido T. Camacho, Ben-Jiang Ma, Yingying Li, Julie M. Decker, Gary J. Nabel, David C. Montefiori, Beatrice H. Hahn, Bette T. Korber, Feng Gao, Barton F. Haynes, A group M consensus envelope glycoprotein induces antibodies that neutralize subsets of subtype B and C HIV-1 primary viruses. Virology. ,vol. 353, pp. 268- 282 ,(2006) , 10.1016/J.VIROL.2006.04.043
Lidija Persic, Andy Roberts, Jane Wilton, Antonino Cattaneo, Andrew Bradbury, Hennie R Hoogenboom, An integrated vector system for the eukaryotic expression of antibodies or their fragments after selection from phage display libraries Gene. ,vol. 187, pp. 9- 18 ,(1997) , 10.1016/S0378-1119(96)00628-2
J. S. Babcook, K. B. Leslie, O. A. Olsen, R. A. Salmon, J. W. Schrader, A novel strategy for generating monoclonal antibodies from single, isolated lymphocytes producing antibodies of defined specificities. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 93, pp. 7843- 7848 ,(1996) , 10.1073/PNAS.93.15.7843
Alicia D. Volkheimer, J. Brice Weinberg, Bethany E. Beasley, John F. Whitesides, Jon P. Gockerman, Joseph O. Moore, Garnett Kelsoe, Barbara K. Goodman, Marc C. Levesque, Progressive immunoglobulin gene mutations in chronic lymphocytic leukemia: evidence for antigen-driven intraclonal diversification. Blood. ,vol. 109, pp. 1559- 1567 ,(2007) , 10.1182/BLOOD-2006-05-020644
Elisabetta Traggiai, Stephan Becker, Kanta Subbarao, Larissa Kolesnikova, Yasushi Uematsu, Maria Rita Gismondo, Brian R Murphy, Rino Rappuoli, Antonio Lanzavecchia, An efficient method to make human monoclonal antibodies from memory B cells: potent neutralization of SARS coronavirus Nature Medicine. ,vol. 10, pp. 871- 875 ,(2004) , 10.1038/NM1080